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Registro completo
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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
11/07/2022 |
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Actualizado : |
02/12/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
GARCÍA-ROCHE, M.; TALMÓN, D.; CAÑIBE, G.; ASTESSIANO, A.L.; MENDOZA, A.; QUIJANO, C.; CASSINA, A.; CARRIQUIRY, M. |
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Afiliación : |
MERCEDES GARCÍA-ROCHE V, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; DANIEL TALMÓN, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; GUILLERMO CAÑIBE, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; ANA LAURA ASTESSIANO, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay.; ALEJANDRO FRANCISCO MENDOZA AGUIAR, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CELIA QUIJANO, Centro de Investigaciones Biomédicas (CEINBIO) and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, 11900, Montevideo, Uruguay.; ADRIANA CASSINA, Centro de Investigaciones Biomédicas (CEINBIO) and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, 11900, Montevideo, Uruguay.; MARIANA CARRIQUIRY, Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, 12900, Montevideo, Uruguay. |
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Título : |
Differential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Journal of Dairy Science, July 2022, Volume 105, Issue 7, Pages 5723-5737. OPEN ACCESS. doi: https://doi.org/10.3168/jds.2021-21358 |
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ISSN : |
0022-0302 |
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DOI : |
10.3168/jds.2021-21358 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 30 September 2021, Accepted 17 March 2022, Available online 20 May 2022, Version of Record 22 June 2022. -- Corresponding author: García-Roche, M.; Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; email:mercedesg@fagro.edu.uy -- LICENSE: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). -- Funding: The authors thank all the staff of the Dairy Unit of the Experimental Station ?La Estanzuela? (Semillero, Uruguay) for their support in animal handling, as well as Ezequiel Jorge-Smeding (Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay) and Irene Benoit (Centro de Investigaciones Biomédicas and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo) for support in the analysis of blood metabolites. MGR conceived and designed laboratory experiments, performed field and laboratory experiments, analyzed the results, and wrote the original draft and revised versions of the manuscript. MC conceptualized and designed field experiment, conceived and designed laboratory experiments, supervised field and laboratory experiments and results analyses, obtained funding and administrated the project, and reviewed and edited the manuscript. CQ conceived, designed, and supervised laboratory experiments, and reviewed and edited the manuscript. AC conceived, designed, and supervised laboratory experiments. AM conceptualized and designed the field experiment and obtained funding. DT, ALA, and GC performed field and laboratory experiments. All authors reviewed the manuscript and approved the final version of the manuscript. M. García-Roche was supported by Comisión Académica de Posgrados (Montevideo, Uruguay) fellowship BDDX_2018_1#49004502. D. Talmón was supported by Agencia Nacional de Investigación e Innovación (ANII) fellowship POS_NAC_2017_1_141266. A. Cassina and C. Quijano were partially funded by grants of the Espacio Interdisciplinario-Centros, Universidad de la República (UdelaR; Montevideo, Uruguay) 2015. A. Cassina was also supported by the grant Comisión Sectorial de Investigación Científica (CSIC) grupos I+D 2014 (767). The project was funded by CSIC of the UdelaR CSIC I+D 2018 ID 103 to M. Carriquiry and C. Quijano, as well as by ANII INNOVAGRO 2018: FSA_1_2018_1_152220 to M. Carriquiry and A. Cassina. A. Mendoza received funding from the project PL_21_0_00 of the Instituto Nacional de Investigación Agropecuaria (Semillero, Uruguay). The authors have not stated any conflicts of interest. |
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Contenido : |
ABSTRACT:
The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could account for the increased persistency of North American cows, especially in a pasture-based system. In addition, we observed an augmented mitochondrial density in New Zealand cows, which could be related to feed efficiency mechanisms. In sum, our results contribute to the elucidation of hepatic molecular mechanisms in dairy cows in production systems with higher inclusion of pastures. © 2022 American Dairy Science Association. MenosABSTRACT:
The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could ... Presentar Todo |
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Palabras claves : |
Dairy cows; Gluconeogenesis; Grazing; Holstein-Friesian; Intermediary metabolismg. |
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Thesagro : |
VACAS LECHERAS. |
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Asunto categoría : |
-- |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/16559/1/J.-Dairy-Sci.-105p.57235737-Mendoza.pdf
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Marc : |
LEADER 05614naa a2200313 a 4500
001 1063411
005 2022-12-02
008 2022 bl uuuu u00u1 u #d
022 $a0022-0302
024 7 $a10.3168/jds.2021-21358$2DOI
100 1 $aGARCÍA-ROCHE, M.
245 $aDifferential hepatic mitochondrial function and gluconeogenic gene expression in 2 Holstein strains in a pasture-based system.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 30 September 2021, Accepted 17 March 2022, Available online 20 May 2022, Version of Record 22 June 2022. -- Corresponding author: García-Roche, M.; Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; email:mercedesg@fagro.edu.uy -- LICENSE: This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). -- Funding: The authors thank all the staff of the Dairy Unit of the Experimental Station ?La Estanzuela? (Semillero, Uruguay) for their support in animal handling, as well as Ezequiel Jorge-Smeding (Departamento de Producción Animal y Pasturas, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay) and Irene Benoit (Centro de Investigaciones Biomédicas and Departamento de Bioquímica, Facultad de Medicina, Universidad de la República, Montevideo) for support in the analysis of blood metabolites. MGR conceived and designed laboratory experiments, performed field and laboratory experiments, analyzed the results, and wrote the original draft and revised versions of the manuscript. MC conceptualized and designed field experiment, conceived and designed laboratory experiments, supervised field and laboratory experiments and results analyses, obtained funding and administrated the project, and reviewed and edited the manuscript. CQ conceived, designed, and supervised laboratory experiments, and reviewed and edited the manuscript. AC conceived, designed, and supervised laboratory experiments. AM conceptualized and designed the field experiment and obtained funding. DT, ALA, and GC performed field and laboratory experiments. All authors reviewed the manuscript and approved the final version of the manuscript. M. García-Roche was supported by Comisión Académica de Posgrados (Montevideo, Uruguay) fellowship BDDX_2018_1#49004502. D. Talmón was supported by Agencia Nacional de Investigación e Innovación (ANII) fellowship POS_NAC_2017_1_141266. A. Cassina and C. Quijano were partially funded by grants of the Espacio Interdisciplinario-Centros, Universidad de la República (UdelaR; Montevideo, Uruguay) 2015. A. Cassina was also supported by the grant Comisión Sectorial de Investigación Científica (CSIC) grupos I+D 2014 (767). The project was funded by CSIC of the UdelaR CSIC I+D 2018 ID 103 to M. Carriquiry and C. Quijano, as well as by ANII INNOVAGRO 2018: FSA_1_2018_1_152220 to M. Carriquiry and A. Cassina. A. Mendoza received funding from the project PL_21_0_00 of the Instituto Nacional de Investigación Agropecuaria (Semillero, Uruguay). The authors have not stated any conflicts of interest.
520 $aABSTRACT: The objective of this study was to assess hepatic ATP synthesis in Holstein cows of North American and New Zealand origins and the gluconeogenic pathway, one of the pathways with the highest ATP demands in the ruminant liver. Autumn-calving Holstein cows of New Zealand and North American origins were managed in a pasture-based system with supplementation of concentrate that represented approximately 33% of the predicted dry matter intake during 2017, 2018, and 2019, and hepatic biopsies were taken during mid-lactation at 174 ± 23 days in milk. Cows of both strains produced similar levels of solids-corrected milk, and no differences in body condition score were found. Plasma glucose concentrations were higher for cows of New Zealand versus North American origin. Hepatic mitochondrial function evaluated measuring oxygen consumption rates showed that mitochondrial parameters related to ATP synthesis and maximum respiratory rate were increased for cows of New Zealand compared with North American origin. However, hepatic gene expression of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and pyruvate dehydrogenase kinase was increased in North American compared with New Zealand cows. These results altogether suggest an increased activity of the tricarboxylic cycle in New Zealand cows, leading to increased ATP synthesis, whereas North American cows pull tricarboxylic cycle intermediates toward gluconeogenesis. The fact that this occurs during mid-lactation could account for the increased persistency of North American cows, especially in a pasture-based system. In addition, we observed an augmented mitochondrial density in New Zealand cows, which could be related to feed efficiency mechanisms. In sum, our results contribute to the elucidation of hepatic molecular mechanisms in dairy cows in production systems with higher inclusion of pastures. © 2022 American Dairy Science Association.
650 $aVACAS LECHERAS
653 $aDairy cows
653 $aGluconeogenesis
653 $aGrazing
653 $aHolstein-Friesian
653 $aIntermediary metabolismg
700 1 $aTALMÓN, D.
700 1 $aCAÑIBE, G.
700 1 $aASTESSIANO, A.L.
700 1 $aMENDOZA, A.
700 1 $aQUIJANO, C.
700 1 $aCASSINA, A.
700 1 $aCARRIQUIRY, M.
773 $tJournal of Dairy Science, July 2022, Volume 105, Issue 7, Pages 5723-5737. OPEN ACCESS. doi: https://doi.org/10.3168/jds.2021-21358
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Registro original : |
INIA La Estanzuela (LE) |
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 | Acceso al texto completo restringido a Biblioteca INIA Treinta y Tres. Por información adicional contacte bibliott@inia.org.uy. |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
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Fecha actual : |
15/01/2021 |
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Actualizado : |
15/01/2021 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
-- - -- |
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Autor : |
ABREO, E.; VALLE, D.; GONZÁLEZ, A.; ALTIER, N. |
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Afiliación : |
EDUARDO RAUL ABREO GIMENEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DIANA VALLE LOPEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ANDRÉS GONZÁLEZ, Universidad de la República, Montevideo, Uruguay. Facultad de Química. Laboratorio de Ecología Química.; NORA ADRIANA ALTIER MANZINI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Control of damping-off in tomato seedlings exerted by Serratia spp. strains and identification of inhibitory bacterial volatiles in vitro. |
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Fecha de publicación : |
2021 |
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Fuente / Imprenta : |
Systematic and Applied Microbiology. April 2021, Volume 44, Issue 2, art. 126177. Doi: https://doi.org/10.1016/j.syapm.2020.126177 |
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DOI : |
10.1016/j.syapm.2020.126177 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 19 October 2020 / Revised 23 December 2020 / Accepted 23 December 2020 / Available online 2 January 2021. |
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Contenido : |
Serratia marcescens can be a plant growth promoting bacteria (PGPB) and an opportunistic human and plant pathogen. We have identified and characterized strains of related species of Serratia and evaluated their biological control of damping-off of tomato seeds caused by Pythium cryptoirregulare. Serratia ureilytica, S. bockelmannii and S. nevei were identified by phylogenetic analysis of partial gyrB gene sequence and average nucleotide identity (ANI). Tomato seeds inoculated with S. ureilytica ILBB 145 showed higher germination percentage and reduced damping-off in greenhouse experiment resembling a commercial operation, and volatiles produced by this strain caused the nearly complete inhibition in vitro of P. cryptoirregulare. Analysis of volatile organic compounds (VOCs) showed that ILBB 145 produced dimethyl disulfide (DMDS), which can partially account for this inhibition. Serratia bockelmannii ILBB 162 performance against damping-off was intermediate and the inhibition of P. cryptoirregulare in vitro was lower and explained by volatile and diffusible metabolites. Both strains augmented DMDS production in the presence of P. cryptoirregulare, suggesting this compound may play a role in the context of interspecific competition. Serratia nevei ILBB 219 showed the lowest inhibition of P. cryptoirregulare in vitro, no DMDS production, and no biocontrol in planta. Draft genomes of the three strains were annotated and individual genes and biosynthesis gene clusters were identified in relation with the observed phenotypes. We report S. ureilytica ? a low risk species- with activity as a biological control agent and DMDS produced by this bacterial species putatively involved in seed and seedling protection against P. cryptoirregulare. MenosSerratia marcescens can be a plant growth promoting bacteria (PGPB) and an opportunistic human and plant pathogen. We have identified and characterized strains of related species of Serratia and evaluated their biological control of damping-off of tomato seeds caused by Pythium cryptoirregulare. Serratia ureilytica, S. bockelmannii and S. nevei were identified by phylogenetic analysis of partial gyrB gene sequence and average nucleotide identity (ANI). Tomato seeds inoculated with S. ureilytica ILBB 145 showed higher germination percentage and reduced damping-off in greenhouse experiment resembling a commercial operation, and volatiles produced by this strain caused the nearly complete inhibition in vitro of P. cryptoirregulare. Analysis of volatile organic compounds (VOCs) showed that ILBB 145 produced dimethyl disulfide (DMDS), which can partially account for this inhibition. Serratia bockelmannii ILBB 162 performance against damping-off was intermediate and the inhibition of P. cryptoirregulare in vitro was lower and explained by volatile and diffusible metabolites. Both strains augmented DMDS production in the presence of P. cryptoirregulare, suggesting this compound may play a role in the context of interspecific competition. Serratia nevei ILBB 219 showed the lowest inhibition of P. cryptoirregulare in vitro, no DMDS production, and no biocontrol in planta. Draft genomes of the three strains were annotated and individual genes and biosynthesis gene clusters were identi... Presentar Todo |
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Palabras claves : |
BGC; BIOCONTROL; INHIBIDORES; OOMYCETE; PLÁNTULAS; SERRAWETTINS; VOCs. |
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Asunto categoría : |
-- |
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Marc : |
LEADER 02728naa a2200265 a 4500
001 1061670
005 2021-01-15
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1016/j.syapm.2020.126177$2DOI
100 1 $aABREO, E.
245 $aControl of damping-off in tomato seedlings exerted by Serratia spp. strains and identification of inhibitory bacterial volatiles in vitro.$h[electronic resource]
260 $c2021
500 $aArticle history: Received 19 October 2020 / Revised 23 December 2020 / Accepted 23 December 2020 / Available online 2 January 2021.
520 $aSerratia marcescens can be a plant growth promoting bacteria (PGPB) and an opportunistic human and plant pathogen. We have identified and characterized strains of related species of Serratia and evaluated their biological control of damping-off of tomato seeds caused by Pythium cryptoirregulare. Serratia ureilytica, S. bockelmannii and S. nevei were identified by phylogenetic analysis of partial gyrB gene sequence and average nucleotide identity (ANI). Tomato seeds inoculated with S. ureilytica ILBB 145 showed higher germination percentage and reduced damping-off in greenhouse experiment resembling a commercial operation, and volatiles produced by this strain caused the nearly complete inhibition in vitro of P. cryptoirregulare. Analysis of volatile organic compounds (VOCs) showed that ILBB 145 produced dimethyl disulfide (DMDS), which can partially account for this inhibition. Serratia bockelmannii ILBB 162 performance against damping-off was intermediate and the inhibition of P. cryptoirregulare in vitro was lower and explained by volatile and diffusible metabolites. Both strains augmented DMDS production in the presence of P. cryptoirregulare, suggesting this compound may play a role in the context of interspecific competition. Serratia nevei ILBB 219 showed the lowest inhibition of P. cryptoirregulare in vitro, no DMDS production, and no biocontrol in planta. Draft genomes of the three strains were annotated and individual genes and biosynthesis gene clusters were identified in relation with the observed phenotypes. We report S. ureilytica ? a low risk species- with activity as a biological control agent and DMDS produced by this bacterial species putatively involved in seed and seedling protection against P. cryptoirregulare.
653 $aBGC
653 $aBIOCONTROL
653 $aINHIBIDORES
653 $aOOMYCETE
653 $aPLÁNTULAS
653 $aSERRAWETTINS
653 $aVOCs
700 1 $aVALLE, D.
700 1 $aGONZÁLEZ, A.
700 1 $aALTIER, N.
773 $tSystematic and Applied Microbiology. April 2021, Volume 44, Issue 2, art. 126177. Doi: https://doi.org/10.1016/j.syapm.2020.126177
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